|
KMID : 0383820110700060482
|
|
Tuberculosis and Respiratory Diseases
2011 Volume.70 No. 6 p.482 ~ p.489
|
|
|
Effect of Apocynin on Acute Lung Injury in Rats Given Interleukin-1￥a Intratracheally
|
|
Lee Young-Man
|
|
|
Abstract
|
|
|
Background: Based on the assertion that apocynin diminishes acute lung injury (ALI) by inhibition of NADPH oxidase, the effect of apocynin was tested in interleukin-1α (IL-1)-induced ALI in rats.
Materials and Methods : IL-1 was insufflated into the trachea of Sprague-Dawley rats to induce ALI, and apocynin (8 mg/kg) was given intravenously for inhibition of NADPH oxidase. In addition, we determined whether apocynin inhibited generation of superoxide anions from isolated human neutrophils. Five hours after IL-1 instillation, lung injury parameters, expression of cytosolic phospholipase A2 (cPLA2) by cells from bronchoalveolar lavage (BAL), an index of oxidative stress in lung tissues (γ-glutamyltranspeptidase, activity), and ultrastructure of alveolar type II (AT II) cells were evaluated.
Results: Apocynin decreased the generation of free radicals from phorbol myristate (PMA)-activated neutrophils in vitro, but did not ameliorate ALI. IL-1 induced enhancement of the expression of cPLA2 on neutrophils was not altered by apocynin.
Conclusion:Apocynin induced suppression of the generation of superoxide anions from neutrophils by inhibition of NADPH oxidase does not attenuate IL-1-induced ALI in rats.
|
|
|
KEYWORD
|
|
|
apocynin, NADPH Oxidase, Acute Lung Injury, Neutrophils
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|
|